Three main types of reproduction are found in chlorophyceae, i. Effects of culture conditions on the growth and reproduction. Zoospores n new gametophyte n new gametophyte n asexual reproduction zoospores n diploid 2 n haploid n haploid n gametes zygote 2 n haplontic and diplontic life cycles reduce one of the generations to a minimum the zygote is the only diploid part of the life cycle. Polymorphic behavior of ulva lactuca chlorophyta in axenic culture. By displaying few distinctive morphological characters, some taxonomical identifications are difficult and the genus is under a conundrum. Tamil nadu coast from rameswaram to kanyakumari tamil nadu has a geographical extent of 1, 30,058 m. Zones of inhibition for escherichia coli, bacillus cereus, bacillus subtilis, klebsiella pneumoniae, staphylococcus aureus, and pseudomonas aeruginosa ranged from 4. Gcms analysis of methanolic extract of ulva lactuca was also recorded. Page designed through the cooperative efforts of interagency itis teams. Ulva is a common component of marine intertidal flora in australia with many species frequently observed along the queensland coastline. Find, read and cite all the research you need on researchgate. The present study is an attempt to understand the unique adaptation of ulva lactuca tolerating periodic desiccationsubmergence cycle using a holistic approach of untargeted metabolomics. Apr 22, 2016 the greater potency of the ulva lactuca compared to the other species was thought to be due to a higher mineral concentration of metals such as copper, zinc, silver and mercury, which have innate antibacterial activity. In this respect ulva lactuca l, a green macro algae and sometimes known as sea lettuce has been well studied and documented.
A valuable resource submit manuscript j anal pharm res 2017, 61. Fruiting margins of ulva lactuca thallus are characterized by a higher content of carotenoids, as well as by higher respiratory and lower photosynthetic activity, than the corresponding vegetative thallus. Antibacterial compounds were extracted using eight solvents, and consistent activity against staphylococcus aureus, bacillus subtilis and methicillinresistant mr s. The alga is a very interesting one for, besides having advanced parenchymatous thalloid type of vegetative body fig. The vegetative growth of ulva lactuca was studied to determine if the growth rate of the alga is driven by infradian rhythmicity. Petri plates were prepared by pouring 20 ml of sabourauds dextrose agar and allowed to solidify for the use in susceptibility test against bacteria. Analysis of metal contents of seaweed ulva lactuca from. Antibacterial activity of ulva fasciata against multidrug oesistant bacterial ptrains m.
In contrast, little seaweed is eaten in western world, especially in europe. In some parts of britain and asia, seaweed is consumed by humans and livestock as it is considered valuable to human nutrition. Materials and methods sample collection the seaweeds kappaphycus alvarezii and ulva lactuca were collected from thoothukudi seashore, tamil nadu, india. The isolated bacterias were subjected to antibacterial activity of ulva lactuca seaweed extract. Phillips, 1990 and has been extensively investigated in u. Antimicrobial activity of three ulva species collected from some. The effect of sea lettuce ulva lactuca mats on sediment. Phytochemical constituents and bioscreening activities of. The bioaccumulation of minerals and heavy metals in u. Pdf seaweeds are known to exhibit various antimicrobial. In addition, they rank among the main marine groups used in a wide range of commercial applications. Metabolic regulatory oscillations in intertidal green. The phycocolloid present in ulva species is used in jam preparation.
Transplantation and isotopic evidence of the relative. Keywords ulva lactuca green alga, antifungal activity, seasonal variation, chemical analyses, gas chromatography. Ulva or sea lettuce species are some of the most abundant. Antimicrobial activity of extracts from macroalgae ulva. Facile green synthesis of zinc oxide nanoparticles using. Screening of antibacterial and antifungal activity of. In the life cycle of the green algae ulva, the phasw that produces male and female gametes is known as a gametophyte. Chapter 11 benthic microorganisms, seaweeds, and seagrasses. Application of the ulva pertusa bioassay for a toxicity.
Insights into life cycle patterns, spore formation. Thiomarinol a 60 is a rare marine natural product isolated from the bacterium alteromonas rava and belongs to a family of pyrancontaining pseudomonic acids. Some studies gave a value of 32% for protein content of ulva lactuca, but this rate appears to be a seasonal high value. Bauer a, miguel rocha d, zenilda laurita bouzon c, marcelo. Dnabarcoding and species identification for some saudi. In this study, ulva rigida was cultivated under two levels of. The following points highlight the top sixteen economic importance of algae. The effect of sea lettuce ulva lactuca mats on sediment quality in three prince edward island estuaries january, 1999 kendall r. Kappaphycus alvarezii extracts against human pathogenic. Other species belonging to the green seaweeds are also consumed as sea vegetables, for example, species such as ulva lactuca or ulva pertusa which are commercially available under the name of sea lettuce. A polysaccharide compound, isolated from ulva lactuca, has been shown to have considerable antiviral effects, reducing replication. Research article the protective role of ulva lactuca. The genus also includes the species previously classified under the genus enteromorpha, the former members of which are known under the common name green nori.
Algae grown with manure demonstrated equal growth rates to algae grown with standard f2medium. Ulva are thin flat green algae growing from a discoid holdfast that may reach 18 cm or more in length, though generally much less, and up to 30 cm across. The overall objective of the present study was to ascertain the antimicrobial activities of the enteromorpha intestinalis and ulva lactuca extract against selected multidrug resistant bacteria. Research article role of ulva lactuca extract in alleviation. The species identification revealed that the specimens samples 1,2,3,4,5,6,7 and 8 belongs to padina pavonica, turbinaria gracilis, carpomitra costata, pterocladiella capillacea, cladostephus spongiosus, ulva lactuca, sporochnus comosus and sargassum muticum, respectively. The sea lettuces comprise the genus ulva, a group of edible green algae that is widely distributed along the coasts of the worlds oceans. Steady state osmotic adaptation in ulva lactuca springerlink.
It is quite eatable and since it is green in color contains chlorophyll, which is a precursor for any organism to be autotrophic. Organic extracts of ulva conglobatadisplayed neuroprotective and antiinflammatory effects on murine hippocampal and microglial cells 21. Submitted in partial fulfilment of the requirements for. Ulva lactuca basic alternation of generations between sporophyte and gametophyte stages. Alternation of generations is defined as the alternation of multicellular diploid and haploid forms in the organisms life cycle, regardless of whether or not these forms are freeliving. This kelp has a twophase life cycle that alternates between the large. Species of the genus ulva are common in anthropogenically disturbed areas and have been reported as the cause of green tides in many areas of the world. Research article role of ulva lactuca extract in alleviation of salinity stress on wheat seedlings waelm.
Environmental change management faculty of science. Antibacterial activity of ulva fasciata against multidrug. Sayed 2 botany department, faculty of science, fayoum university, fayoum, egypt. The crude extracts of the seaweed ulva reticulata was also screened. Ulva reticulata and ulva lactuca against human pathogens. It is found clinging to rocks and other debris near the shore. Together, results of the disc diffusion and mic assays confirm the antimicrobial activity of u. Although summarized as a simple alternation of isomorphic generations, the haplodiplontic life cycle of many ulva species is generally more complex foyn, 1958. Antimicrobial and phytochemical screening of seaweeds.
Ulva species have been considered as ideal candidates for carbon capture, bioremediation and biofuel production. Chloroform and ethyl acetate fractions of ulbi, ii and iii were prepared and screened for invitro antimicrobial activities and also against kleibesella pnumoniae infected mice invivo. In this respect ulva lactuca l, a green macro algae and. This choice of food grade solvent and green extraction method exemplifies some of the approaches that can be used in the. Ulva lactuca as feed ingredient in giant freshwater prawn macrobrachium rosenbergii n. Ulva lactuca in particular for the use as organic fertiliser will be discussed. D glucopyranosylstigmasta5,25dien isolated from ulva lactuca, showed topical antiinflammatory activity when tested on the mouse ear oedema assay 20. Antifungal activity and seasonal variation of green alga ulva. In vitro exposure of ulva lactuca linnaeus chlorophyta to.
This class of compounds is under significant scrutiny in the search for improved, less toxic analogs of mupirocin, a commercially successful topical antimicrobial medication for skin infections. Ulva lactuca extracts had significantly lower antibacterial activity. Ulva usually multiply bymeans of fragments which are accidentally produced from a thallus. Silver nanoparticles synthesis by ulva fasciata had very high antibacterial activity against the common pathogens bacteria of human and fish33. Seaweeds are getting importance in various fields ranging from food to medical. Ulva lactuca is a thin flat green algae growing from a discoid holdfast. The zoospores are formed in ordinary vegetative cell by the dividing up of protoplast. Influence of temperature on the infradian growth rhythm in. Potential using of ulvan polysaccharide from ulva lactuca. Ulva pertusa which is frequently consumed under the name aonori by japanese people shows a high protein level 26% of dry weight table 9.
The reproduction cycle of ulva lactuca is given in figure 4. Ulva lactuca is a rich source of iron and vitamin c and has been consumed by people in scotland chapman and chapman, 1980. The type species within the genus ulva is ulva lactuca, lactuca being latin for lettuce. The aim of this chapter is to gain insights from literature on the suitability of ulva lactuca as organic fertiliser. The marine representatives are limited to relatively few orders and are common in. Effects of culture conditions on the growth and reproduction of gut weed, ulva intestinalis linnaeus ulvales, chlorophyta rapeeporn ruangchuay 1, sofhanee dahamat, anong chirapat2, and masahiro notoya3 1 department of technology and industry, faculty of science and technology. The water lettuce ulva lactuca is green to dark green. Krishnamoorthy1 1department of botany, annamalai university, annamalainagar 608 002, tamil nadu, india. Cultivation of ulva lactuca with manure for simultaneous bioremediation and biomass production. Hydrolyzed ulva lactuca extract skin deep cosmetics. The influence of temperature on the infradian rhythm of growth was also investigated. The results also showed that the highest antifungal activity was recorded in winter season while the lowest one was in summer.
Phytochemical screening was assessed by chemical analysis, while the coagulatory effect was assessed by measuring prothrombin time pt and activated partial thromboplastin time aptt. Preliminary investigation into the induction of reproduction in ulva spp. Marine macroalgae like ulva lactuca are increasingly recognized as potential. However, higher protein contents were recorded for certain red seaweeds. Feb 19, 2015 regulation and manipulation of the life cycle. Such rnaseq experiments can also provide essential information about profiles of gene expression during the ulva life cycle and development. Antibacterial activity of silver nanoparticles using ulva. In vitro and in vivo antimicrobial activity of ulva lactuca. In the life cycle of ulva lactuca both the haploid and diploid phases include a from ecol 182 r at university of arizona. Cultivation of ulva lactuca with manure for simultaneous. The production of antimicrobial compounds by british marine algae. Kappaphycus alvarezii extracts against human pathogenic bacteria. Photosynthesis in the male margin was also characterized by a longer induction period than was observed in the vegetative thallus by the polarographic method. The ncbi blast results details for the 8 seaweeds rbcl sequences.
Many of its nutrients include iron, protein 15 percent, iodine, vitamins a, b1, and c and trace elements. Several species of green, brown and red reproduction, settlementbiofouling and feeding algae with luxuriant growth occur along the southern deterrents 5. Of the approximately 4,500 7000 species of green algae, 90% are restricted to the freshwater environment. Escherichia coli was more resistant bacteria in a comparison to others because it showed totally resistant against some algae. The effects of hyper and hyposaline stresses on the levels of various inorganic and organic solutes inulva lactuca have been recorded. Many seaweeds exhibit photoperiodic control over the reproduction of germ cells.
The membrane is two cells thick, soft and translucent and grows attached without a stipe to rocks by a small discshaped holdfast. In vitro and in vivo antimicrobial activity of ulva. Marine algae derivatives seem to be good candidates in novel, antibacterial drug discovery 11. In contrast, growth at 15 or 20 provoked a predominance of the 2day cycle and induced reproduction. Nov 17, 2011 this study aimed to optimize an extraction and separation procedure to obtain a concentrated fraction with antibacterial activity from the macroalga ulva lactuca. Pdf antimicrobial activity of ulva reticulata and its endophytes. Antimicrobial action of compounds from marine seaweed mdpi.
The zoospores are formed in ordinary vegetative cell by the. Freefloating ulva lactuca was collected late summer 2010 in the inner parts of limfjorden, a eutrophic. Vegetative multiplication also takes place by means of the proliferation of perennial holdfast. Silver nanoparticles synthesis by ulva fasciata have toxic against cancer1,31,32. Asexual reproduction takes place by means of quadriflagellate zoospores. Hydrolyzed ulva lactuca extract is a hydrolysate of ulva lactuca extract q. Alan brindo abstract the evaluation of seaweed, ulva lactuca as feed ingredients in giant freshwater prawn macrobrachium rosenbergii was carried out by incorporating raw and fermented ulva lactuca at three levels, 10 %, 20 % and 30 % in diets. In some species, such as the alga ulva lactuca, the diploid and haploid forms are indeed both freeliving independent organisms, essentially identical in. The ether extracts of laurencia johnstonii, ulva lactuca and dictyota flabellata were more active in comparison with buthanol extracts against s. Spirogyra crassa showed very poor antibacterial activity where its antifungal activity was moderate. When sea lettuce is soft, stir in well beaten eggs and boil for a few seconds then remove from heat. Impacts on aspects of reproduction and development in three marine invertebrates. Transplantation and isotopic evidence of the relative effects of ambient and internal nutrient supply on the growth of ulva lactuca.
Ulva lactuca is known by its common name sea lettuce. Ulva fasciata has potent therapeutic value and it is used for synthesis of silver nanoparticles31. The potential of liquid manure as sole nutrient source for cultivation of ulva lactuca was investigated with the perspective of utilizing the produced biomass for feed andor energy. In the life cycle of ulva lactuca both the haploid and. The potential of bionanofertilizer is to be assessed for the whole plant life cycle up to quality and. However, little is known regarding the effects of simultaneous ocean warming, acidification and eutrophication on these capacities. Hosted by the usgs core science analytics and synthesis. Facile green synthesis of zinc oxide nanoparticles using ulva lactuca seaweed extract and its evaluation of photocatalytic, antibiofilm and larvicidal activity. Exploring the potential for using seaweed ulva lactuca as organic. The green alga specimens ulva lactuca linnaeus were collected in april 2009 during a low tide at alqusayr the protective role of ulva lactuca against genotoxic and biochemical effects induced by.
Sea lettuce and gut weed are intertidal marine algae that display a. Preserving your articles for eternity is a free service that lets you to preserve your original articles for eternity. Studies were undertaken to establish the most optimal conditions for growing ulva in. Preliminary investigation into the induction of reproduction.
1212 789 1116 1134 925 601 194 1438 1340 39 524 908 135 1416 555 609 1221 1615 810 1352 528 885 127 702 204 1516 861 770 567 935 267 1438 246 782 1344 976 547 1092 1497 139 1062 1258 344 1255 538 1401